Detection of Cucumber green mottle mosaic virus in low-concentration virus-infected seeds by improved one-step pre-amplification RT-qPCR

Table 5 Detection of CGMMV in 33 batches of seeds using three detection methods

Samples	Number^b (batch)	Subsamples^c/batch (m)	Number of positive results^d (Positive number/total number^e)
Samples	Number^b (batch)	Subsamples^c/batch (m)	Method A	Method B	Method C
Zucchini seeds	13	20	3(20/20;20/20;19/20)	3(20/20;11/20;2/20)	2(20/20;7/20)
Cucumber seeds	15	20	0	0	0
Pumpkin Seeds	5	20	0	0	0

CGMMV Cucumber green mottle mosaic virus LOD limit of detection
^aThe three methods: (A) pre-amplification RT-qPCR; (B) universal RT-qPCR [14, 24]; (C) DAS–ELISA
^bNumber of seed batches
^cEach batch of seeds is divided into 20 subsamples, m = 20
^dNumber of positive results from 20 subsamples
^eRatio of the number of positive results to the number of subsamples

ISSN: 1746-4811