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Fig. 4 | Plant Methods

Fig. 4

From: Optimization of immune receptor-related hypersensitive cell death response assay using agrobacterium-mediated transient expression in tobacco plants

Fig. 4

Effect of leaf position on TIRRPS4-mediated autoimmune and RPS4/RRS1-mediated effector triggered HR cell death in tobacco plants. A TIRRPS4-dependent autoimmune activity is different for each leaf position. N. benthamiana leaf was transiently agro-infiltrated with 35S::TIRRPS4-GFP/35S::GFP or inactive mutant 35S::TIRRPS4(SH/AA)-GFP/35S::GFP or 35S::TIRRRS1-GFP/35S::GFP or 35S::TIRRPS4(SH/AA)-GFP/inactive mutant 35S::TIRRRS1(SH/AA)-GFP at leaf positions #4-#7. The HR cell death was confirmed on the 3 days. B RPS4/RRS1-mediated effector triggered HR cell death exhibited differently infiltrated leaf position in N. benthamiana. Agro-infiltration was performed with transiently coexpressed 35S::RRS1-R-HF/35S::RPS4-Myc with 35S::GFP or 35S::AvrRps4-GFP or non-functional mutant 35S::AvrRps4KRVY/AAAA-GFP at leaf positions #4-#7 in N. benthamiana. The HR cell death for leaf positions #4-#5 is enlarged. C RPS4/RRS1-mediated effector triggered HR cell death exhibited differently infiltrated leaf position in N. tabacum. Agro-infiltration was performed with transiently coexpressed 35S::RRS1-R-HF/35S::RPS4-Myc with 35S::GFP or 35S::AvrRps4-GFP or non-functional mutant 35S::AvrRps4KRVY/AAAA-GFP at leaf positions #4-#5 in N. tabacum. HR cell death for leaf positions #4-#5 is enlarged. The HR cell death was confirmed on the 4 days

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Plant Methods

ISSN: 1746-4811