Fig. 3From: Optimization of immune receptor-related hypersensitive cell death response assay using agrobacterium-mediated transient expression in tobacco plantsEffect of leaf position and humidity on RPS4/RRS1-mediated autoimmunity in N. benthamiana. A The autoimmune activity of RPS4-mediated RRS1-cCFP or RRS1-nCerulean at leaf positions #4 and #5 was different at low humidity. The positions of the leaves are numbered from top to bottom. 35S::RRS1-R-cCFP or 35S::RRS1-R-cCFP/35S::RPS4-Myc or 35S::RRS1-R-nCerulean or 35S::RRS1-R-nCerulean/35S::RPS4-Myc were transiently co-expressed in N. benthamiana leaf positions #4 and #5. The HR cell death was confirmed on the 5Â days. B The autoimmune activity of RPS4-mediated RRS1-cCFP or RRS1-nCerulean at leaf positions #4 and #5 was suppressed by high humidity. 35S::RRS1-R-cCFP/35S::RPS4-Myc or 35S::RRS1-R-nCerulean/35S::RPS4-Myc were transiently co-expressed in N. benthamiana leaf positions #4 and #5. The HR cell death was confirmed on the 5Â daysBack to article page