Fig. 1

Visualization of organellar microcapture in Carya ovata under transmitted light (A, C) and fluorescence microscopy (B, D). A An empty micropipette prior to insertion into the target parenchyma cell—note that the amyloplasts (a) in the target cell and adjacent cells are readily visible, but the nucleus (arrow) is less distinctly resolvable. B The same frame as A, with the DAPI-stained target nucleus (arrow) easily discernible, and the parenchyma cell walls exhibiting lignin autofluorescence. C Amyloplasts and the target nucleus successfully aspirated into the micropipette. D The same frame as C, confirming that the nucleus is among the captured organelles. Scale bars 20 µ