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Fig. 3 | Plant Methods

Fig. 3

From: A procedure for Dex-induced gene transactivation in Arabidopsis ovules

Fig. 3

Induction in ovules and anthers. a–h Dex induction of the following lines: (a) RPS5a >  > GUS, (b, c, h) RPS5a >  > H1.1-RFP (d) RPS5a >  > YFP-Δkinesin, (e–g) SPL >  > GUS. (i–i’) Mock treatment of an RPS5a >  > YFP-Δkinesin line, in ovule primordia (a–f, i, stages are indicated according to the nomenclature [22]) or anthers from flower stage 12 (g, h). Time post induction: 24 h (a), 16 h (b–i). Confocal images: partial projections (b, d), full projection (c-inset, h), combined orthogonal sections (c); scale bar: 20 μm for all except c, 10 μm

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