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Fig. 2 | Plant Methods

Fig. 2

From: A procedure for Dex-induced gene transactivation in Arabidopsis ovules

Fig. 2

Induction of a variety of constructs with distinct subcellular targets. (a) RPS5a >  > YFP-Δkinesin; b RPS5a >  > nls3xmVenus. c RPS5a >  > H1.1-RFP (d) RPS5a >  > GUS. Ovule primordia (a–d) were dissected from flowers stage 9, sampled 24 hpi (a, d) or 5 dpi (b, c). e RT-PCR showing FIE and MET1 expression levels in whole flower buds stage 6–10 (pooled) [43] expressing the inducible SPL >  > amiRFIE or SPL >  > amiRMET1, respectively. ***: 2-tailed t-test, P < 0.001. See Additional file 1 for raw and processed data. Scale bar = 10 µm

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