Fig. 2

Chromatin marks analysis by X-ChIP method for peach mesocarp tissue. Histone modification analysis on chromatin extracted from FAN mesocarp tissue at 83, 104, and 111 DAFB. The ‘TSS around-’ (dark bars, questioned with primer set A) and ‘gene body-’ (white bars, questioned with primer set C) subregions of FLESHY genomic locus, were investigated by real-time PCR quantification on ChIPed DNA immunoprecipitated with α-H3K4me3 (a), and α-H3K27me3 (b). Data are reported as a percentage of chromatin input (% IP), normalized on background signal (No Ab serum control sample, measured by omitting antibody during ChIP procedure). Three PCR repetitions for each ChIP assay. Standard errors are reported. Asterisks indicate statistically significant changes: * = p ˂ 0.05, ** = p ˂ 0.01. DAFB Days after full bloom