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Fig. 1 | Plant Methods

Fig. 1

From: An efficient chromatin immunoprecipitation (ChIP) protocol for studying histone modifications in peach reproductive tissues

Fig. 1

Crosslinking efficiency and physical shearing chromatin analyses. a Bud and mesocarp tissues were crosslinked in buffers containing increasing amounts of formaldehyde (0, 1, and 3%). Samples were subjected or not to a reverse crosslinking phase (decrosslinked sample + and −, respectively), and DNA was isolated using phenol/chloroform extraction as described in “Materials and methods” section. While DNA is efficiently isolated from samples that were not crosslinked (lanes indicated with 0%), a decrosslinking procedure is required for the isolation of DNA from cross-linked samples (with 1% indicating the relative concentration of formaldehyde used in testing analyses, which resulted in a better yield of signal). b Chromatin shearing check after the application of 60% amplitude with several 10 s shearing rounds (25 for FB and 15 for FM) followed by a reverse crosslinking phase and a DNA isolated using phenol/chloroform extraction

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