Fig. 5
From: Design and fabrication of an improved dynamic flow cuvette for 13CO2 labeling in Arabidopsis plants
Incorporation of 13C into intermediates of primary metabolism following 13CO2 exposure, as determined by LCMS/MS analysis of labeled plant tissue. Labeling of triose phosphate (A), 2C-methyl-d-erythritol-2,4-cyclodiposphate (MEcDP, B), and isopentenyl and dimethylallyl diphosphate (IDP + DMADP, C) are shown. Each time point represents a separate experiment where an individual Arabidopsis plant was first adapted, then labeled with air containing 400 μL L−1 13CO2, and finally flash frozen. Labeling assays ranging from 5 s to 50 min (n = 19 plants) were performed to examine the linearity of short labeling assays and the initial slope (rate) of label incorporation by fitting the earliest time points to a linear regression. The embedded graphs inside each panel show the full time-course fitted to an exponential rise to maximum. D Overview of the metabolic sequence from the Calvin-Benson cycle (CBC) to IDP and DMADP in the chloroplast indicates their expected order of labeling. Hollow arrows indicate multiple steps and OP signifies a phosphate group and OPP a pyrophosphate. GAP, d-glyceraldehyde-3-phosphate; DXP, 1-deoxy-d-xylulose-5-phosphate