Fig. 4

Performance evaluation of the dynamic flow cuvette. A The replacement time of the atmosphere in the cuvette head space was measured at different flow rates by making a step change to a ¹³CO₂ containing air and observing the decay of the IRGA signal. B Calculated half-life of air in the cuvette at different flow rates. The data were fitted to a power function in Excel. All air flow experiments were performed with a plant inside the cuvette. C Resistance of the cuvette to temperature changes at increasing light intensity. The water bath set point was fixed as light intensity varied to assess the ability of the block to maintain temperature across a light intensity gradient. Temperatures reflect the abaxial leaf surface inside the cuvette using a thermocouple