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Table 3 Effect of the different induction styles and nutrient components on the root regeneration of S.m.-SC

From: Development of a highly efficient virus-free regeneration system of Salvia miltiorrhiza from Sichuan using apical meristem as explants

Induction style

Group

Basel medium

IBA

(mg/L)

NAA

(mg/L)

Sucrose

(g/L)

Rooting rate (%)

Main Root number

Main Root Length(cm)

Main Root diameter(mm)

Solid medium

M0

1/2 MS

10

38.46 ± 4.36 f

2.33 ± 0.58 b

3.47 ± 0.93 f

0.70 ± 0.06 b

M1

1/2 MS

0.5

0.5

10

53.33 ± 6.67 e

1.67 ± 0.58 c

3.41 ± 1.01 f

0.67 ± 0.09 b

M2

1/2 MS

1.0

10

74.42 ± 3.70 c

5.33 ± 1.15 a

4.48 ± 1.46 e

0.87 ± 0.03 b

M3

1/2 MS

1.0

10

80.00 ± 6.67 b

4.00 ± 0.84 a

4.14 ± 0.75 f

0.91 ± 0.05 a

Improved hydroponics

H0

0

60.98 ± 2.10 d

2.33 ± 0.58 b

6.71 ± 0.19 e

0.40 ± 0.06 d

H1

1/2 MS

0

75.50 ± 4.41 c

2.67 ± 0.58 b

9.89 ± 0.35 b

0.53 ± 0.05 c

H2

1/2 MS

0.5

0.5

0

83.33 ± 3.30 a

3.67 ± 0.58 b

7.64 ± 1.01 d

0.33 ± 0.03 d

H3

1/2 MS

1.0

0

72.22 ± 1.92 c

5.00 ± 0.10 a

10.86 ± 0.77 a

0.44 ± 0.07 c

H4

1/2 MS

1.0

0

87.88 ± 6.67 a

4.00 ± 1.34 a

8.47 ± 1.17 c

0.55 ± 0.09 c

  1. a For each group (M0-H3) and rooting affection rooting rate, main root number, length, and diameter means separated by difference letter are significantly different at P < 0.05 according to Duncan's test. Number of regeneration buds per group was twelve; three replication of each group were laid