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Table 2 Effect of medium components in combination with MS medium on the survival of apical meristem

From: Development of a highly efficient virus-free regeneration system of Salvia miltiorrhiza from Sichuan using apical meristem as explants

Group

Medium components

Survival Rate (%)

Growing status

NAA (mg/L)

GA3 (mg/L)

BA (mg/L)

Agar (g/L)

Sucrose (g/L)

B0

0

0

0

8.5

30

34.09 ± 4.69 d

B1

0.1

0.1

0

8.5

30

40.48 ± 4.41 d

B2

0.1

0.1

0.25

8.5

30

67.35 ± 4.24 b

Vitrified bud

B3

0.1

0.1

0.50

8.5

30

75.47 ± 2.53 a

Partial health buds

B4

0.1

0.1

1.00

8.5

30

58.00 ± 2.81 c

Vitrified buds, callus formed

B5

0.1

0.1

0.50

7.5

20

75.47 ± 2.89 a

Emaciated, vitrified buds

B6

0.1

0.1

0.50

7.5

30

77.36 ± 2.61 a

Vigorous bud

B7

0.1

0.1

0.50

8.5

20

62.50 ± 3.37 b

Emaciated bud

B8

0.1

0.1

0.50

9.5

20

53.06 ± 5.65 c

Emaciated bud

B9

0.1

0.1

0.50

9.5

30

69.44 ± 4.34 b

Vitrified bud

  1. Number of apical meristem inoculated per group was twelve; three replication of each group were laid. Data were collected after 4 weeks. Different letters indicate significant differences among mediums for each group as determined by Duncan’s multiple range test followed by one-way ANOVA at the p < 0.05 significant level