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Table 1 Virus detection and symptom observation of diseased plants of S.m.-SC by Das-ELISA test

From: Development of a highly efficient virus-free regeneration system of Salvia miltiorrhiza from Sichuan using apical meristem as explants

Plant

Virus

Symptom of diseased plant

TMV

CMV

To MV

TSWV

D1

P*

N

N

N

(1) Mosaic;

(2) Albino in young leaf;

(3) Vein clearing;

(4) Branches wither prematurely

D2

P*

N

N

N

D3

P*

N

N

N

D4

P*

N

N

N

D5

P*

N

N

N

D6

P*

N

N

N

D7

P*

N

N

N

D8

P*

N

N

N

D9

P*

N

N

N

D10

N

P*

N

N

(1) Yellow mosaic spreading from vein;

(2) Bulk dry spot

D11

N

P*

N

N

D12

P*

P*

N

N

Mosaic;

Black spots;

Branches wither prematurely

D13

P*

P*

N

N

D14

P*

P*

N

N

D15

N

N

N

N

(1) Withered leave;

(2) Necrotic spot, mottled

D16

N

N

N

N

D17

N

N

N

N

  1. P*: positive, N: negative; all plant samples were determined at least twice