Fig. 7
Established virus-free regeneration system for S.m.-SC. Starting from explant disinfection and cultivation in B6 media (MS basal medium supply 0.5 mg/L 6-BA, 0.1 mg/L NAA and 0.1 mg/L GA3), light condition (24 h dark/d), and medium component (MS basal medium supply 2 mg/L TDZ and 0.1 mg/L NAA) for callus induction of secondary petiole tissue. After adventitious bud induction and proliferation in B6 media, the shoots were rooting and acclimatization was improved hydroponics (culture liquwid component: 1/2 MS basal medium supply 1 mg/L NAA). The completely regenerated shoots were subjected to hardening-seeding in nutrient soil and transplanted in soil. The red box in diseased plant shows the terminal buds of S.m.-SC. The black arrow indicates the peeled primary apical meristem. The red circle in directly regenerated bud shows the trimming part of secondary petiole. The virus detection of regenerated plants: hen the seedlings grow to 8-10 cm height, collect directly regenerated seedlings and adventitious bud leaves for virus detection