Fig. 5

A Bud organogenesis of S.m.-SC through secondary callus on B6 media under the light condition with 16 h light/8 h dark. B Regenerated buds' induction rate of callus. C Induction number of regeneration buds per callus. SM: Buds induced from secondary apical meristem, the growth status of buds: long and narrow leaf, transparent yellow green, vitrification (Small image on the lower right shows buds organogenesis in red circle). SL: buds induced from secondary leaf, the growth status of buds: small buds slowly product from the spherical embryoid growing from the surface bulge of massy callus (arrow indicates bud organogenesis). SP: buds induced from secondary petiole, the growth status of buds: vigorous, green leaf stretch (arrow indicates bud organogenesis). Different letters indicate significant differences among mediums for each group as determined by Duncan’s multiple range test followed by one-way ANOVA at the p < 0.05 significant level, and all of materials was repeated three times