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Table 4 The components and levels of factors used for walnut micropropagation and measured traits mean values applied to characterize it

From: Predictive modeling of Persian walnut (Juglans regia L.) in vitro proliferation media using machine learning approaches: a comparative study of ANN, KNN and GEP models

Walnut

Culture medium

Nutrients (× DKW basal medium) and PGRs concentrations

PR

CW (g)

STN

Vit

F1

F2

F3

F4

F5

F6

F7

F8

F9

NH4NO3, CaNO3, ZnNO3

KNO3

K2SO4

MgSO4, MnSO4, CuSO4

KH2PO4, H3BO3, Na2MoO4

FeEDDHA

Thiamine, Nicotinic acid, Glycine

BAP (mg/l)

TDZ, IBA (mg/l)

Chandler

              
 

1

0.5

2

2

2.5

0.5

0.5

0.5

2

2

9.5 ± 0.18

0.79 ± 0.01

30.5 ± 0.9

17.3 ± 0.32

 

2

1.25

2

0

1.5

1.5

2

0.5

0.5

1.25

8.1 ± 0.44

0.20 ± 0.01

20.3 ± 0.6

11.7 ± 0.36

 

3

2

2

1

0.5

2.5

1.25

0.5

1.25

0.5

6.75 ± 0.2

0.49 ± 0.02

27.2 ± 0.4

13.1 ± 0.44

 

4

2

1

0

2.5

1.5

0.5

2

2

1.25

10 ± 0.32

0.89 ± 0.05

31.2 ± 1.1

12.8 ± 0.29

 

5

1.25

0

1

2.5

1.5

2

0.5

1.25

2

8.6 ± 0.18

0.97 ± 0.06

33.3 ± 1

21.2 ± 0.45

 

6

2

0

2

1.5

2.5

1.25

0.5

2

1.25

7.3 ± 0.7

1.09 ± 0.06

30.1 ± 0.4

29.1 ± 0.51

 

7

1.25

2

0

1.5

2.5

0.5

1.25

1.25

2

6.2 ± 0.31

0.89 ± 0.01

30.7 ± 0.7

16.5 ± 0.32

 

8

0.5

2

2

2.5

1.5

1.25

1.25

0.5

0.5

10.5 ± 1.1

0.28 ± 0.01

20.2 ± 0.9

0

 

9

0.5

1

1

1.5

0.5

0.5

0.5

1.25

1.25

6.3 ± 0.3

0.5 ± 0.01

21 ± 0.8

0

 

10

2

2

1

0.5

1.5

0.5

2

0.5

2

10.7 ± 0.9

0.99 ± 0.03

36.1 ± 1.1

11.5 ± 0.42

 

11

0.5

0

0

0.5

1.5

1.25

1.25

1.25

1.25

11.7 ± 1

0.5 ± 0.01

33.1 ± 0.9

5.5 ± 0.32

 

12

1.25

1

2

0.5

1.5

2

0.5

2

0.5

10.5 ± 0.3

0.49 ± 0.01

39.3 ± 1.1

10.7 ± 0.36

 

13

1.25

1

2

0.5

1.5

2

0.5

2

0.5

10 ± 0.32

0.49 ± 0.01

34.3 ± 0.8

6.7 ± 0.35

 

14

0.5

0

0

0.5

0.5

0.5

0.5

0.5

0.5

4.8 ± 0.3

0.24 ± 0.01

20.6 ± 0.7

4.6 ± 0.37

 

15

1.25

0

1

2.5

0.5

1.25

2

0.5

1.25

7.6 ± 0.18

0.2 ± 0.01

12.6 ± 0.6

9.8 ± 0.39

 

16

0.5

0

0

0.5

2.5

2

2

2

2

5.7 ± 0.31

0.89 ± 0.01

31.6 ± 0.8

27.1 ± 0.45

 

17

2

1

0

2.5

0.5

2

1.25

1.25

0.5

9.7 ± 0.28

0.13 ± 0.01

13.3 ± 0.3

13.2 ± 0.33

 

18

0.5

1

1

1.5

2.5

2

2

0.5

0.5

6.7 ± 0.25

0.18 ± 0.01

11.3 ± 0.3

3.8 ± 0.29

 

19

1.25

1

2

0.5

0.5

1.25

2

1.25

2

8 ± 0.32

0.52 ± 0.01

27.1 ± 0.5

6.8 ± 0.25

 

20

1.25

1

2

0.5

2.5

0.5

1.25

0.5

1.25

7.3 ± 0.41

0.20 ± 0.01

15 ± 0.6

6.8 ± 0.39

 

21

0.5

1

1

1.5

1.5

1.25

1.25

2

2

5.8 ± 0.3

0.73 ± 0.01

22 ± 0.5

9.3 ± 0.32

 

22

1.25

0

1

2.5

2.5

0.5

1.25

2

0.5

9.1 ± 0.29

0.2 ± 0.01

15.7 ± 1

7.1 ± 0.29

 

23

2

2

1

0.5

0.5

2

1.25

2

1.25

5.8 ± 0.3

1.1 ± 0.03

21.8 ± 0.6

19.5 ± 0.32

 

24

0.5

2

2

2.5

2.5

2

2

1.25

1.25

21.8 ± 0.7

0.09 ± 0.01

11.1 ± 0.7

3.1 ± 0.12

 

25

2

0

2

1.5

0.5

2

1.25

0.5

2

13.6 ± 0.3

0.98 ± 0.01

18.1 ± 0.8

10.7 ± 0.45

 

26

2

1

0

2.5

2.5

1.25

0.5

0.5

2

12.1 ± 0.3

0.8 ± 0.02

13.6 ± 0.6

9.7 ± 0.44

 

27

2

0

2

1.5

1.5

0.5

2

1.25

0.5

9.7 ± 0.31

0.51 ± 0.01

11.6 ± 0.6

29 ± 0.46

 

DKW

1

1

1

1

1

1

1

1

0.1

7.12 ± 0.35

0.3 ± 0.03

10.75 ± 0.45

0

Rayen

              
 

1

0.5

2

2

2.5

0.5

0.5

0.5

2

2

8.5 ± 0.42

0.92 ± 0.03

33.8 ± 0.8

21.7 ± 0.59

 

2

1.25

2

0

1.5

1.5

2

0.5

0.5

1.25

7.7 ± 0.31

0.28 ± 0.02

25.6 ± 1.1

14 ± 0.26

 

3

2

2

1

0.5

2.5

1.25

0.5

1.25

0.5

6.3 ± 0.37

0.48 ± 0.01

49.5 ± 1.4

15.7 ± 0.36

 

4

2

1

0

2.5

1.5

0.5

2

2

1.25

8 ± 0.37

0.94 ± 0.02

25.3 ± 1.5

16.8 ± 0.29

 

5

1.25

0

1

2.5

1.5

2

0.5

1.25

2

7.7 ± 0.31

0.99 ± 0.02

27.2 ± 1.3

27.7 ± 0.45

 

6

2

0

2

1.5

2.5

1.25

0.5

2

1.25

8 ± 0.32

0.79 ± 0.03

29.6 ± 0.6

31.3 ± 0.54

 

7

1.25

2

0

1.5

2.5

0.5

1.25

1.25

2

5.8 ± 0.29

1.07 ± 0.04

31.2 ± 1

17.3 ± 0.37

 

8

0.5

2

2

2.5

1.5

1.25

1.25

0.5

0.5

6.3 ± 0.26

0.31 ± 0.01

21.8 ± 1.1

6 ± 0.46

 

9

0.5

1

1

1.5

0.5

0.5

0.5

1.25

1.25

6.3 ± 0.46

0.52 ± 0.01

27 ± 0.4

6.2 ± 0.36

 

10

2

2

1

0.5

1.5

0.5

2

0.5

2

7 ± 0.53

1.05 ± 0.04

33.1 ± 1.2

15 ± 0.37

 

11

0.5

0

0

0.5

1.5

1.25

1.25

1.25

1.25

8 ± 0.42

0.45 ± 0.01

28.5 ± 0.4

8 ± 0.26

 

12

1.25

1

2

0.5

1.5

2

0.5

2

0.5

8.2 ± 0.49

0.17 ± 0.01

28 ± 0.6

10.6 ± 0.46

 

13

1.25

1

2

0.5

1.5

2

0.5

2

0.5

9 ± 0.46

0.39 ± 0.01

27.3 ± 0.9

9.2 ± 0.36

 

14

0.5

0

0

0.5

0.5

0.5

0.5

0.5

0.5

6.1 ± 0.39

0.27 ± 0.01

23.3 ± 0.5

4 ± 0.26

 

15

1.25

0

1

2.5

0.5

1.25

2

0.5

1.25

7 ± 0.26

0.29 ± 0.01

21.7 ± 1.3

12.1 ± 0.29

 

16

0.5

0

0

0.5

2.5

2

2

2

2

6 ± 0.46

0.72 ± 0.02

29 ± 1.5

31.8 ± 0.44

 

17

2

1

0

2.5

0.5

2

1.25

1.25

0.5

7.3 ± 0.37

0.26 ± 0.01

18.1 ± 1.4

16.1 ± 0.37

 

18

0.5

1

1

1.5

2.5

2

2

0.5

0.5

6.3 ± 0.53

0.31 ± 0.01

27.2 ± 1.3

5 ± 0.32

 

19

1.25

1

2

0.5

0.5

1.25

2

1.25

2

7.5 ± 0.5

0.46 ± 0.01

27.8 ± 0.5

7.1 ± 0.29

 

20

1.25

1

2

0.5

2.5

0.5

1.25

0.5

1.25

7.5 ± 0.32

0.29 ± 0.01

20.1 ± 0.6

6.7 ± 0.25

 

21

0.5

1

1

1.5

1.5

1.25

1.25

2

2

6 ± 0.26

0.94 ± 0.02

27.1 ± 0.7

10.7 ± 0.36

 

22

1.25

0

1

2.5

2.5

0.5

1.25

2

0.5

6.5 ± 0.32

0.16 ± 0.01

22 ± 0.9

6 ± 0.37

 

23

2

2

1

0.5

0.5

2

1.25

2

1.25

6.6 ± 0.25

0.91 ± 0.03

27.2 ± 0.9

22.5 ± 0.5

 

24

0.5

2

2

2.5

2.5

2

2

1.25

1.25

7 ± 0.31

0.17 ± 0.01

27.5 ± 0.6

6 ± 0.26

 

25

2

0

2

1.5

0.5

2

1.25

0.5

2

10.2 ± 0.28

0.55 ± 0.02

21.6 ± 0.8

9.3 ± 0.41

 

26

2

1

0

2.5

2.5

1.25

0.5

0.5

2

7.2 ± 0.36

0.94 ± 0.03

29.5 ± 0.7

11.6 ± 0.37

 

27

2

0

2

1.5

1.5

0.5

2

1.25

0.5

8.3 ± 0.37

0.46 ± 0.01

27.3 ± 0.3

35 ± 0.46

 

DKW

1

1

1

1

1

1

1

1

0.1

6.25 ± 0.4

0.5 ± 0.06

23.75 ± 0.59

16.12 ± 1.1

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Plant Methods

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