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Fig. 6 | Plant Methods

Fig. 6

From: A cationic lipid mediated CRISPR/Cas9 technique for the production of stable genome edited citrus plants

Fig. 6

Targeted mutagenesis of NPR3. T7 Endonuclease I (T7EI) assay targeting CsNPR3 indicating cleavage products from targeted mutagenesis (A). Positive sign ( +) is used to indicate T7EI reaction by incubate the annealed PCR product at 37 ºC for 15 min. The untreated samples are marked with negative sign (−). Sanger sequencing chromatograms of four selected lines. Arrows point to the editing site and the modifications are indicated alongside (B)

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Plant Methods

ISSN: 1746-4811