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Fig. 5 | Plant Methods

Fig. 5

From: A cationic lipid mediated CRISPR/Cas9 technique for the production of stable genome edited citrus plants

Fig. 5

Development of genome edited transgenic plants following lipofectamine-mediated plasmid delivery into protoplast. A Colonies of developed embryos, (B) EGFP-expressing embryos. A germinating EGFP expressing seedling visualized under white light (C) and the same seedling exhibiting EGFP expression under an epi-fluorescence stereomicroscope (D). E Regenerated plants in tissue culture medium before micrografting and (F) Micrografted plant well acclimated in the greenhouse. Inset shows a section of the leaf exhibiting EGFP fluorescence as visualized under the dissecting microscope fitted with a NIGHTSEA fluorescence adapter. Scale bar indicates 1 cm in length

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Plant Methods

ISSN: 1746-4811