Skip to main content
Fig. 8 | Plant Methods

Fig. 8

From: Orthogonal control of gene expression in plants using synthetic promoters and CRISPR-based transcription factors

Fig. 8

Design and characterization of a ratiometric circuit. A OSC3-5 contains YFP which is inducible by ACC (pATF-1), while BFP and RFP are constitutively expressed under the control of pATF-3 via the constitutive expression of gRNA-3. B Fluorescence microscope images showing Agrobacterium mediated transient expression of the ratiometric OCS construct (OCS3-5) in Nicotiana benthamiana leaves with or without 10 μM ACC. Scale bars: 200 μm C qPCR quantification of YFP, BFP and RFP shows that YFP is induced after the treatment with ACC while the expression of BFP and RFP remains unchanged before or after ACC induction. Error bars: S.D. (n = 4, independent replicates). An asterisk indicates statistical significance in a student t-test (P < 0.05)

Back to article page

Plant Methods

ISSN: 1746-4811