Fig. 7From: Orthogonal control of gene expression in plants using synthetic promoters and CRISPR-based transcription factorsDegree of orthogonality of synthetic promoters. A OCS circuit containing all three synthetic promoters (pATF-1, pATF-3 and pATF-4) driving three different reporter genes namely YFP, BFP and RFP respectively with a single gRNA expressed one at a time under the control of U6 promoter. B Fluorescence microscope images showing Agrobacterium mediated transient expression of OCS constructs in Nicotiana benthamiana leaves. Scale bars: 200 μm C As observed from the fluorescence images, only the specific gRNA:pATF pair is active, thus demonstrating that the synthetic promoters are mutually orthogonal Relative integrated density of each fluorescence signal (shown in panel B). Integrated density was measured by image J software and normalized to the highest value. Error bars: S.D. (n = 3, independent replicates). Asterisks indicate statistical significance in a student t-test (P < 0.05)Back to article page