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Fig. 6 | Plant Methods

Fig. 6

From: Orthogonal control of gene expression in plants using synthetic promoters and CRISPR-based transcription factors

Fig. 6

Characterization of an ethylene inducible orthogonal control system. A OCS1-9 circuit (gRNA-1 is expressed by ethylene inducible EBS promoter) B Time course fluorescence microscope images showing Agrobacterium mediated transient expression of OCS1-9 in Nicotiana benthamiana leaves after induction with 10 μM ACC. Scale bars: 200 μm C and D qPCR quantification of gRNA-1 (C) and YFP (D) expression before and after induction with ACC, where both show similar levels of induction demonstrating that the relative change in gRNA-1 expression (ethylene induction) results in the differential activation from the pATF-1 promoter. Error bars: S.D. (n = 3, independent replicates), Asterisks indicate statistical significance in a student t-test (P < 0.05)

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Plant Methods

ISSN: 1746-4811