Fig. 2

Characterization of reporter constructs assembled. A Fluorescence microscope images showing Agrobacterium mediated transient expression of YFP, BFP, RFP and GFP under the control of 35S promoter into Nicotiana benthamiana leaves. Images on the left are from non-infiltrated leaves (negative control) captured using the appropriate filter at same exposure and gain settings as was used for the positive images on the right (Material and Methods). B Relative integrated density of each fluorescence signal (shown in panel A). Integrated density was measured using image J software and normalized to that of a non-infiltrated control (con). Error bars: S.D. (n = 3, independent replicates). Asterisks indicate statistical significance in a student t-test (P < 0.05). C Luminescence reporter luciferase expression shown by Agrobacterium mediated transient expression of luciferase in Nicotiana benthamiana leaves. Left half of the leaf was not infiltrated with Agrobacterium. D. Fluorescence microscope images showing Agrobacterium mediated transient expression of YFP under MAS promoter in Nicotiana benthamiana leaves. Image on the left is the brightfield image for the same construct