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Table 1 Measurements of cell wall layers

From: Super-resolution imaging of Douglas fir xylem cell wall nanostructure using SRRF microscopy

 

Wet

Dry

Autofluorescence

Acriflavin

Rhodamine B

Autofluorescence

Acriflavin

Rhodamine B

SRRF

408

552

144

456

384

144

432

624

144

288

480

144

384

480

120

360

288

144

408

480

120

360

288

120

360

456

144

360

288

144

624

456

264

432

312

120

480

624

240

336

336

120

360

360

240

384

288

144

648

384

120

480

384

120

576

456

168

336

360

120

468a

487a

170d

379e

341f

132g

CLSM

480

936

528

552

600

408

624

744

576

408

864

480

456

624

600

432

600

384

504

720

432

432

840

432

648

600

648

432

552

432

744

672

864

504

576

384

624

840

888

456

552

480

480

480

912

456

552

456

696

504

408

528

576

432

648

624

552

504

600

384

590b

674c

641c

470a

631c

427a

SEM

337

     

300

     

337

     

375

     

337

     

337

     

347

     

319

     

356

     

291

     

334f

     
  1. Measurements of the width of the compound middle lamella using a paired comparison of SRRF and CLSM (average projection) at matched locations for wet and dry conditions compared to reference measurements by SEM. Methods were significantly different as were interactions between image type and method, p << 0.001. Means with different letters are significantly different for p = 0.05