Fig. 4
From: Super-resolution imaging of Douglas fir xylem cell wall nanostructure using SRRF microscopy
a A comparison of photobleaching during the 255-s exposure for SRRF imaging. Bleaching rate (grey-levels/second) is comparable for autofluorescence, acriflavin, and rhodamine B. b Noise levels as measured by pixel variance in a representative region of interest of cell wall from a single confocal image with no averaging. Autofluorescence and acriflavin images show significantly more noise compared to rhodamine B. c Resolution determined by FRC analysis comparing CLSM (average projection) with SRRF and SRRF + Denoising