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Fig. 6 | Plant Methods

Fig. 6

From: Ribozyme-mediated CRISPR/Cas9 gene editing in pyrethrum (Tanacetum cinerariifolium) hairy roots using a RNA polymerase II-dependent promoter

Fig. 6

Pyrethrum hairy root transformation workflow using leaf explants. At 0 days: Agrobacterium infection. Agrobacterium cell density with an OD600 = 0.5. Co-cultivation began. At 2 days: Co-cultivation was over. Roots’ induction began. At 22 days: Roots’ induction was over. Screening of roots transformed with Ri began. At 29 days: Screening of roots transformed with Ri was over. Screening of roots transformed with Ti began. At 49 days: Screening of roots transformed with Ti was over. Sub-cultivation began

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Plant Methods

ISSN: 1746-4811