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Fig. 2 | Plant Methods

Fig. 2

From: Ribozyme-mediated CRISPR/Cas9 gene editing in pyrethrum (Tanacetum cinerariifolium) hairy roots using a RNA polymerase II-dependent promoter

Fig. 2

Transformation of pyrethrum mediated by A. rhizogenes harboring the pBI121 vector. a Resistant roots grown on selective medium containing kanamycin (10 mg/L) for 20 days. b Resistant hairy roots were excised and transferred onto selective medium containing kanamycin (10 mg/L) to propagate for approximately 60 days. c Transgenic roots stained with X-gluc reagent. d PCR detection of NPT II in samples from 60-day-old resistant roots. M, marker; +, bacterial plasmid DNA control; −, wild type plant control. e Magnified image of dyed roots from Line 5. Numbers represent different lines, as follows: 1, Line 1; 2, Line 2; 3, Line 3; 4, Line 4; 5, Line 5. Scale bars in a–c and e, 5.0 mm

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Plant Methods

ISSN: 1746-4811