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Fig. 1 | Plant Methods

Fig. 1

From: Ribozyme-mediated CRISPR/Cas9 gene editing in pyrethrum (Tanacetum cinerariifolium) hairy roots using a RNA polymerase II-dependent promoter

Fig. 1

Induction of pyrethrum hairy roots. a Twenty days after transformation, roots emerged from the infected leaf explants. b Root tips were placed on culture medium and culture dishes oriented vertically to ensure that the root tips were upward. c After a week of cultivation, two different root types appeared: one exhibiting geotropism and the other without geotropism. d PCR detection of rol B in the two types of root. M, marker; 1, bacterial plasmid DNA control; 2–4, DNA from roots exhibiting geotropism; 5–7, DNA from roots without geotropism. Scale bars in ac, 1.0 cm

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