Table 1 Abbreviated procedures for the three proposed lysis and DNA extraction methods discussed in this study
From: Simple and efficient isolation of plant genomic DNA using magnetic ionic liquids
Static extraction method |
Pipette 6 µL of MIL or IL onto 40 mg of plant tissue Allow the coated plant tissue to incubate Collect the hydrophobic liquid using a pipette Add 0.3 µL of either the DNA-enriched MIL or IL to the qPCR buffer |
Dispersive extraction method |
Suspend 40 mg of cut-up plant tissue in a Tris-based buffer Add 6 µL MIL to the sample Disperse the sample using a vortex Recover the MIL with a magnet Pipette 0.3 µL MIL into the qPCR buffer |
One-pot PCR method |
Grind plant tissue to a powder Add 1 mg plant tissue, 6 µL of MIL or IL, and the qPCR buffer to a reaction tube |