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Fig. 3 | Plant Methods

Fig. 3

From: Simple and efficient isolation of plant genomic DNA using magnetic ionic liquids

Fig. 3

Amount of DNA recovered from the (green) [P6,6,6,14+][Ni(hfacac)3] and (blue) [P6,6,6,14+][Co(hfacac)3] MIL using the optimized dispersive lysis and DNA extraction method. Buffer volume: 0.5 mL; buffer composition with the [P6,6,6,14+][Ni(hfacac)3] MIL: 2 mM Tris, 50 mM EDTA; buffer composition with the [P6,6,6,14+][Co(hfacac)3] MIL: 2 mM Tris; mass of plant tissue: 40 mg; volume of MIL dispersed: 6 µL; dispersion time for the [P6,6,6,14+][Ni(hfacac)3] MIL: 30 s; dispersion time for the [P6,6,6,14+][Co(hfacac)3] MIL: 60 s. *DNA recovered could not be quantified

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