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Table 1 Success assessment after 6 days post-grafting recovery of grafted Arabidopsis thaliana plantlets

From: Mix-and-match: an improved, fast and accessible protocol for hypocotyl micrografting of Arabidopsis seedlings with systemic ACC responses as a case study

 

Total number of grafted seedlings

Successfully grafted (assessed after 6 days)

Unsuccesfully grafted seedlings

Percentage of success (%)

Number of seedlings developing AR

Not aligned scion and rootstock

First time

 Researcher 1

30

14

7

9

46.6

 Researcher 2

40

21

5

14

52.5

 Researcher 3

40

12

9

19

30

Second time*

 Researcher 1

29

25

1

3

86.2

 Researcher 2

29

27

0

2

93.10

 Researcher 3

57

54

0

3

94.7

  1. The data were collected from grafting experiments of three independent non-trained researchers, and differentiate between the first and the second attempt
  2. Total number of grafted seedlings is indicated, as well as both the successfully and unsuccessfully grafted seedlings (differentiating between the ones that developed adventitious roots (AR) and the ones with poor root-to-scion alignment)
  3. The percentage of success is calculated as successfully grafted seedlings/total number of seedlings
  4. A paired one-tailed T-test was used to identify significant differences between success rates at the first and the second attempt (*p-value > 0.05)
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Plant Methods

ISSN: 1746-4811