Skip to main content
Fig. 3 | Plant Methods

Fig. 3

From: Mix-and-match: an improved, fast and accessible protocol for hypocotyl micrografting of Arabidopsis seedlings with systemic ACC responses as a case study

Fig. 3

Arabidopsis thaliana grafting of wild type Col-0. A Two Whatman cellulose papers with on top two Hybond N membrane strips are soaked in sterile distilled water. They are subsequently placed in a Ø 90 mm round petri dish. Seedlings are transferred on top of the Hybond-N membrane strips. B Seedlings are cut and desired scion-rootstock assemblies are made. C When grafting is finished, moisture content is assessed. The Whatman paper and Hybond-N membrane strips should be well saturated with water (Left). A film around the seedling (Right) indicates excess water, which should be avoided around the hypocotyl

Back to article page