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Table 1 Polypeptides detected in the PSI-mc-s and the NDH complex by MS analysis

From: Qualitative and quantitative evaluation of thylakoid complexes separated by Blue Native PAGE

 

Plant

Protein

M.W

PSI-mc1

PSI-mc2

PSI-mc3

PSI-mc4

NDH

Da

Score

Score

Score

Score

Score

PSI

 A0A3B6UD72

Z. m

psaA

83,055

4357x,a

4362x

1862a,x

1568x

 

 A0A5P8KLV9

Z. m

psaB

82,517

5909x,a

4607x

3917a,x

1866x

 

 A0A5P8KLH3

Z. m

psaC

8893

15414x,a

5503x

6691a

6269x

 

 A0A317YJL6

Z. m

psaD

21,563

41944x,y

 

30676b,a,x,y

  

 B4FAW3

Z. m

psaD

21,563

 

57637y,x

 

18722x

 

 A0A3L6E4J8

Z. m

psaE

14,427

32357x,y

28888y,x

27,812 b,a,x,y

4825x,a,b

 

 A0A096RAV0

Z. m

psaF

24,487

30393y,a

42839y,x

30,393 b,a,x,y

6960x,y

 

 B6U534

Z. m

PsaG

15,304

6320x,a

5675x

5733a,x

1368x

 

 A0A3L6E9J9

Z. m

psaH

14,920

11956x

8266x

20186b,x,y

756a

 

 A0A3L6E041

Z. m

psaK

13,595

3790x

3444x

3790x,a

3697x

 

 A0A3L6DL54

Z. m

psaL

22,240

985x

829x

1183a

2609x

 

 A0A3L6FDJ3

Z. m

psaN

23,369

3461x

 

2576a

  

 A0A3L6F840

Z. m

lhca-P4

29,477

11070x,a,b

9737x

10327a,x,y

  

 A0A3L6EJZ9

Z. m

lhca5

33,854

376a

    

 A0A0F7H1K9

H. b

lhca6

29,515

 

1057y

   

 A0A6M3WH11

C.p

lhca6

28,442

 

1018y

   

NDH chloroplastic

 e-donor binding

  K7TFI4

Z. m

ndhU

25,323

194a

7106x

  

3688x

  A0A3L6GCK8

Z. m

ndhU

25,309

7627x

    

 Subcomplex A

  A0A5P8KLE9

Z. m

ndhH

45,629

12097x,a

12285x

479a

 

7474x

  A0A5P8KLE6

Z. m

ndhI

21,143

27850x,a

13488x

  

15901x

  A0A5P8KLA0

Z. m

ndhJ

18,657

7105x,a

26589x

  

18907x

  A0A5P8KLC5

Z. m

ndhK

27,902

11795x

7443x

  

5595x

  B4FRX4

Z. m

ndhM

23,964

17739x

11325x

  

6239x

  A0A3L6FA99

Z. m

ndhN

23,024

2640x,a

17139x

  

9570x

  A0A3L6DTN1

Z. m

ndhO

16,595

12097x,a

3167x

  

3296x

 Subcomplex B

  A0A3L6GBC8

Z. m

pnsB1

51,403

15044y,x,a,b

13418x,y

4167a,b,x,y

4529x,a,b,y

 

  A0A3L6DA24

Z. m

pnsB2

37,902

32626x,a,b,y

23464y

8554a,b,x,y

2260b,a

 

  B4FWG2

Z. m

pnsB2

37,902

 

27745x

 

15228x

 

  A0A3L6E2Y7

Z. m

pnsB3

20,798

4649x,y

5414x

   

  A0A1D6HK95

Z. m

pnsB4

31,198

  

1985x

  

  A0A1D6HK96

Z. m

ndhB4

20,603

2637y

 

2637b

  

  A0A3L6FDN2

Z. m

pnsB5

25,605

10998y,x

10306x

1864a,x,y

7131x,a,b

 

 Membrane subcomplex

  A0A3B6UD78

Z. m

ndhA

40,346

579a

 

909a

  

  P25706

Z. m

ndhA

40,485

5094x

6331x

  

5854x

  A0A5P8KL97

Z. m

ndhC

13,851

1596x

1606x

   

  A0A5P8KM09

Z. m

ndhD

56,316

3444x,a

4815x

503x,a

985x

 

  A0A5P8KLF2

Z. m

ndhF

82,950

5788x,a

6092x

2561a,x

2077x,a

 

 Lumenal subcomplex

 A0A1D6JYG6

Z. m

pnsL1

25,683

10829y,a,b,x

10571x,y

5036b,a,x

 

6759x,y

 A0A1D6HBD7

Z. m

pnsL2

24,200

27466y,a,b,x

20585x,y

4104b,a,x,y

 

15443x,y

 A0A3L6E7E6

Z. m

pnsL3

25,686

5953y

5382x

2365b,a

1008a

 

 A0A1D6HQR2

Z. m

pnsL3

28,290

 

5957y

 

879b

 
  1. Data were obtained from bands originating from two different isolations and separations. Analysis was performed in maize (mark a) and PSI-NDH (mark b) databases (isolation 1, separation in 4.3–12% BN gel gradient) and maize (mark x) and PSI-NDH (mark y) databases (isolation 2, separation in 4.3–8% BN gel gradient) acquired form UniProtKB. Search results can be found in Additional file 3: MS results 1 (isolation 1) and Additional file 4: MS results 2 (isolation 2). Superscripts show the hits. The highest scores (first superscript marks) are shown. Nomenclature of Shikanai [72] was used in the case of chloroplast NDH. The pnsB1 present in PSI-mc-s but not in the free NDH complex and ndhH corresponds to band A and band 1, respectively, in Fig. 5c
  2. CA: carbonic anhydrase; C.p.: Carica papaya; FS: iron-sulphur protein; FV: flavoprotein; H.b.: Hypseocharis bilobata; su: subunit; Z.m.: Zea mays
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Plant Methods

ISSN: 1746-4811