Fig. 9

Amounts, ratios, and distribution of complexes in untreated and lincomycin treated maize mesophyll thylakoids. Complexes are marked as in Fig. 1a, s: supercomplex, d: dimer, m: monomer. Thylakoids were solubilised (500 µg Chl mL⁻¹) using 1% (w/V) β-DM plus 1% (w/V) digitonin and separated in 4.3–12% BN gel gradient followed by SDS PAGE. a Relative amount of complexes in thylakoids isolated from untreated (white) and lincomycin treated leaves (grey) given as the percentage of controls. Differences were compared using two-way ANOVA with Bonferroni’s multiple comparison test [P < 0.05; significance is indicated by stars (*)]. Inset: Distribution of total band volumes among the complexes. Differences were compared using multiple t-tests [P < 0.05; significance is indicated by stars (*)]. b Ratio of complexes. The PSI/PSII, PSI-LHCI/PSI, and Lhc-m/LHCII-t ratios were calculated from the BN bands, i.e. PSI and PSII contained the bound antennae, while LHCII/PSII was calculated from the SDS gel pattern: all Lhcb spots and PsbB spots were taken into account (see Additional file 2). Differences were compared using multiple t-tests [P < 0.05; significance is indicated by stars (*)]. c Distribution of PSII complexes (PsbB in SDS PAGE pattern to eliminate the effects of different antenna sizes): PSII-d (light grey), PSII-m (dark grey), PSII-s (medium grey). In controls some CP43-less PSII was also present (white on the top). Differences were compared using multiple t-tests [P < 0.05; significance is indicated by stars (*)]. In all cases, n = 2 × 3 (biological × technical)