Fig. 8
From: Qualitative and quantitative evaluation of thylakoid complexes separated by Blue Native PAGE
Characterisation of thylakoid complexes in untreated (Ctrl) and lincomycin treated (LM) maize leaves. Complexes obtained by BN PAGE (a) and CN PAGE (b) separations. Complexes are marked as in Fig. 1a, mc: megacomplexes, *: PSI-LHCII*. Polypeptide patterns of untreated (c) and LM treated (d) thylakoid complexes. Standard proteins: PageRuler™ Plus Prestained Protein Ladder (ThermoFisher Scientific 26619, Lot #00803392). Thylakoids (500 µg Chl mL−1) were solubilised using 1% (w/V) β-DM plus 1% (w/V) digitonin, and complexes were separated in 4.3–12% gel gradients followed by SDS PAGE