Fig. 3
From: Qualitative and quantitative evaluation of thylakoid complexes separated by Blue Native PAGE
Effect of solubilisation protocols on the complex pattern of maize mesophyll thylakoids. a Representative gel patterns of thylakoids (500 µg Chl mL−1) solubilised using different detergents and detergent mixtures (given in % w/V), and separated in 4.3–12% gel gradient. Dig: digitonin, β-DM: n-dodecyl-β-d-maltoside, DS: n-dodecyl-sucrose. b The ratio of the more sensitive bands is given in % of the total PSI, PSII and Lhc amounts, respectively. Complexes are marked as in Fig. 1a. Smear is the bluish green staining at the beginning of the separating gel where bands cannot be noticed clearly given in % of total stain in the lane. Effects of different detergents were compared using one-way ANOVA with Tukey’s multiple comparison test for each group separately [P < 0.05; 6 technical repetitions: 3× solubilisation and separation in 2–2 lanes]. Significant differences are indicated by different letters in each group separately