Fig. 2
From: Qualitative and quantitative evaluation of thylakoid complexes separated by Blue Native PAGE
Proportionality of the thylakoid complexes in BN gels. a, b Band volumes, c ratios of complexes, and d distribution of the different PSII forms as the function of the sample volume applied per lane. Complexes are marked as in Fig. 1a, s: supercomplex, d: dimer, m: monomer, summa: total band volumes/lane. Poplar thylakoids (500 µg Chl mL−1) were solubilised using 2% (w/V) β-DM and separated in 5–12% gel gradient with three technical repetitions. PSI/PSII and LHCII-t/Lhc-m ratios were calculated from BN bands, LHCII/PSII and distribution of PSII among its different forms are calculated from SDS gel patterns: all Lhcb spots and PsbB spots were taken into account (see Additional file 2)