Table 3 Irrigation protocol
From: A glass bead semi-hydroponic system for intact maize root exudate analysis and phenotyping
Days after planting | Time that plants were drip irrigated hours/schedule | Time plants were flooded hours/schedule | Volume supplied—pause (total volume per hour) |
|---|---|---|---|
4 or 5 | 4 h/2–6 p.m | 18 h/6–12 p.m. next day | 30 mL–30 s (3600 mL/h) |
6 | 4 h/12–4 p.m 2 h/6–8 p.m | 2 h/4–6 p.m 16 h/8–12 p.m. next day | 35 mL–40 s (3150 mL/h) |
7 | 4 h/12–4 p.m 4 h/6–8 p.m | 2 h/4–6 p.m 16 h/8 –12 p.m. next day | 35 mL–50 s (2520 mL/h) |
8 | 4 h/12–4 p.m 14 h/8 p.m.–10 a.m. next day | 4 h/4–8 p.m | 35 mL–60 s (2100 mL/h) |
9 | 20 h/2 p.m.–10 a.m. next day | 4 h/10 a.m.–2 p.m | 35 mL–70 s (1800 mL/h) |
10 | 20 h/2 p.m.–10 a.m. next day | 4 h/10 a.m.–2 p.m | 35 mL–70 s (1800 mL/h) |
11 | 20 h/2 p.m.–10 a.m. next day | 4 h/10 a.m.–2 p.m | 35 mL–70 s (1800 mL/h) |
12 | 20 h/2 p.m.–10 a.m. next day | 4 h/10 a.m.–2 p.m | 35 mL–70 s (1800 mL/h) |
13 | 18.5 h/2 p.m.–8:30 a.m. next day | 4 h/10 a.m.–2 p.m | 40 mL–70 s (2057 mL/h) |
14 | Exudates were collected with 1Â mM CaCl2 at 10:30Â a.m | ||
1.5 h/10:30 a.m.–12:00 p.m 18.5 h / 2 p.m.—8:30 a.m. next day | 2 h/12–2 p.m | 40 mL/70 s (2057 mL/h) | |
15 | Exudates were collected with MQ at 10:30Â a.m Roots weighted and scanned afterwards | ||