Fig. 2

Differences in root and shoot phenotypic traits between genotypes. a Root area (AR), number of foreground pixels belonging to the root system. b Rooting depth skeleton (RDS), describes the longest root length. c Skeleton width (SW), which was calculated from the medial axis of the root system. d Number of root tips paths (NRTP), which is the overall number of tips detected in the image. e Mean tip diameter (MTD), f Stem diameter (SD), derived from the medial axis. g Fresh root weight (FRW). h Fresh shoot weight (FSW). Statistical differences detected by All pairs Tukey–Kramer HSD (honest significant difference), α = 0.05. Measurements with different letters within each graph are significantly different. Traits obtained by DIRT using the scanned root images, panels a–f, are represented with arbitrary units given by DIRT. Roots were scanned individually, while root and shoot weight represent the average of the total root or shoot weight divided by the number of plants grown in a single glass tube used to collect exudates. Number of samples used to obtain DIRT parameters AR, RDS, SW, NRTP, MTD, SD: Cize 7 n = 5; Ames 12734, Ames 20140, Ames 27171, NSL 22629 n = 6; Ames 20190 n = 7; PI 57154 n = 8. Number of samples for FRW and FSW: Cize 7, Ames 12734, Ames 20140, Ames 27171, NSL 22629 n = 3; Ames 20190, PI 57154 n = 4. Boxplot: Box, interquartile range (IQR); line inside the box, median; end of the box, upper (Q3) and lower (Q1) quartiles; dots beyond the extreme lines show potential outliers