Development of a controlled-environment assay to induce iron deficiency chlorosis in soybean by adjusting calcium carbonates, pH, and nodulation

Table 2 Main effects of the “Check Genotypes Experiment”

Source of variation	Level	Visual score (1–9 Scale)		atLEAF value		Height (cm)		Root dry mass (g)		Shoot dry mass (g)
Source of variation	Level	Mean	SE	Mean	SE	Mean	SE	Mean	SE	Mean	SE
Genotype	'Corsoy 79'	4.5^a	0.16	32.3^n.s	0.50	11.1^a	0.23	0.62^n.s	0.02	1.1^a	0.04
Genotype	'Dawson'	3.8^b	0.13	31.9^n.s	0.41	12.0^b	0.22	0.65^n.s	0.02	1.2^b	0.04
Nodulation status	Nodulated	3.5^a	0.12	33.7^a	0.37	12.4^a	0.24	0.69^a	0.02	1.3^a	0.04
Nodulation status	Unnodulated	4.8^b	0.16	30.8^b	0.50	10.8^b	0.20	0.58^b	0.02	1.0^b	0.04
pH	6.5	3.9^a	0.19	32.4^ab	0.59	11.7^ab	0.30	0.66^a	0.03	1.2^a	0.06
	7.5	4.1^ab	0.18	33.1^a	0.55	11.8^a	0.28	0.65^ab	0.03	1.2^a	0.05
	8.5	4.5^b	0.18	31.4^b	0.53	11.2^b	0.26	0.59^b	0.03	1.1^b	0.05
CCE%	0%	2.8^a	0.14	36.7^a	0.47	12.8^a	0.31	0.71^a	0.04	1.6^a	0.07
	10%	4.1^b	0.19	32.0^b	0.51	11.5^b	0.34	0.66^ab	0.03	1.1^b	0.05
	20%	4.5^b	0.20	30.6^bc	0.60	11.2^b	0.29	0.62^bc	0.03	1.0^bc	0.05
	30%	5.3^c	0.20	29.9^c	0.67	10.9^b	0.31	0.55^c	0.03	0.9^c	0.04

Main Effects of the Check Genotypes Experiment and means and standard error (S.E.) for each measured trait pooling both runs (n = 6). Significant differences between means within each source of variation were determined using Tukey’s HSD test and are indicated with differing letter superscripts

ISSN: 1746-4811