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Fig. 4 | Plant Methods

Fig. 4

From: High-throughput estimation of allele frequencies using combined pooled-population sequencing and haplotype-based data processing

Fig. 4

The pool obtained allele frequency (P1) for three tested sequencing strategies (WGrS, GBS, MACE) correlated to individual genotyping (KASP). Three different methods were compared on their accuracy for pool sequencing of large pools. A SNP-based pool AF compared to the KASP assay detected allele frequency for WGrS. B Gene haplotype-based pool AF compared to KASP assay for WGrS. C SNP-based pool AF to KASP for GBS. D Gene haplotype-based pool AF compared to KASP assay for GBS. E SNP-based pool AF to KASP for MACE RNAseq. F Gene haplotype-based pool AF compared to KASP assay for MACE RNAseq. The dotted line indicates the optimal match of the individual (KASP assay) and pool sequencing. Each point represents a single locus—for (A, C and E), a locus is a SNP; for B, D and F, a locus is a gene, related to a KASP marker. The colour of the points presents the read coverage of the locus. The red line is a regression through all points

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Plant Methods

ISSN: 1746-4811