Fig. 6
Visualization of mRNA distribution by split FP-based RNA live-cell imaging system. Confocal microscopy of N. benthamiana leaves co-expressing A, E M-FPN and P-FPC without target mRNA; B, F M-FPN and P-FPC with RFPHSL12; or C, D, G, H M-FPN and P-FPC with FTHSL12 RNA. E–H are the magnified images of the rectangle marked area in A–D. Different fluorescence distribution was detected in cells expressing RFPHSL12 or FTHSL12 RNA. G The punctate GFP fluorescence in the cell periphery is indicated by arrowheads. H The GFP fluorescence spot located on nucleus boundary or transported along cytoplasmic strands is indicated by an arrow or arrowhead, respectively. Scale bar = 20 mm in all images