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Fig. 2 | Plant Methods

Fig. 2

From: Development of a split fluorescent protein-based RNA live-cell imaging system to visualize mRNA distribution in plants

Fig. 2

Illustration of split fluorescent protein (FP)-based RNA live-cell imaging system. A MCP or PCP, the coat proteins of MS2 or PP7 bacteriophage, was fused with the N- or C-terminus of split FPs, FPN or FPC, respectively. The FT or RFP mRNA was conjugated with 12 copies of MBS-PBS, the hairpin structure recognized by MCP and PCP, to generate FTHSL12 or RFPHSL12 chimeric RNA. The binding of MCP and PCP with the hairpin structure of MBS-PBS brings two split FPs together to reconstitute the functional FP for indirect visualization of mRNAs in living cells. B Illustration of the constructs of MCP (M) and PCP (P) fused with the N- or C-terminus of FPN or FPC. The nuclear localization sequence (NLS) from Arabidopsis FD was inserted to sequester MCP- or PCP-FP fusion proteins in the nucleus. The constructs were driven by a CaMV35S promoter and had an NOS terminator

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