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Fig. 1 | Plant Methods

Fig. 1

From: Development of a split fluorescent protein-based RNA live-cell imaging system to visualize mRNA distribution in plants

Fig. 1

Background GFP fluorescence disturbs observation of labeled mRNA. A Confocal microscopy of N. benthamiana leaves co-infiltrated with nucleus-localized MCPFD-GFP and FT-SL24, which is the coding sequences of Arabidopsis FLOWERING LOCUS T (FT) conjugated with 24 repeats of stem loop (SL) structures recognized by MCP [13]. The background fluorescence derived from nucleus-localized MCP-GFP interferes with the detection of potential FT mRNA foci (arrows) in the nucleus. Nucleus boundary is outlined with white dots. Bar = 2 μm. Inset, in cells with high accumulation level of MCP-GFP in the nucleus, high nucleus-to-cytoplasmic ratio of GFP fluorescence disturbs detection of FT mRNA foci (arrowheads) proximal to nucleus. Bar = 5 μm. B Roots of Arabidopsis transformant harboring constitutively expressed MCPFD-GFP. Note that in cells with high nucleus-to-cytoplasmic ratio, the background GFP fluorescence may interfere in mRNA localization in the cytosol. Scale bar = 20 mm

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