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Fig. 4 | Plant Methods

Fig. 4

From: A foliar pigment-based bioassay for interrogating chloroplast signalling revealed that carotenoid isomerisation regulates chlorophyll abundance

Fig. 4

Chlorophyll and carotenoid content in young and old leaves of Arabidopsis germplasm that alter cis-carotene biosynthesis. A Chlorophyll b, B Chlorophyll a, C Total chlorophyll, D Lutein, E β-carotene, F Violaxanthin, G Neoxanthin, H Antheraxanthin, I Zeaxanthin, and J Total carotenoids were quantified in young and old leaves of different germplasm. Mean values are displayed with standard error (n = 4) being a representative dataset from two experimental repetitions. Letters denote statistical variation (p < 0.05) within leaf types across different germplasm determined using a Two-Way ANOVA and post-hoc Holm-Sidak multiple comparison. ziso and crtiso are loss of function mutants in ζ-carotene isomerase and carotenoid isomerase, respectively. PSY-OE enables higher PSY activity (35S::AtPSY#23) [46] and 35S::AtCRTISO restored CRTISO activity to the loss-of-function ccr2.1 mutant [16]

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Plant Methods

ISSN: 1746-4811