Stability investigation of air-dried olive ribo nucleic acids for metavirome studies

Table 3 RNA concentrations comparison of various fresh and lyophilized methods in different cultivars

Method of extraction		Cultivar	Concentration (ng/µL)	CV	A260/280	A260/230
Fresh extractions	TRIzol	Amin	562.85	0.48	1.52	01.15
		Meshkat	711.93	0.77	1.73	0.91
		Arbequina	395.26	0.55	1.79	0.95
		Amygdalolia	597.16	0	1.56	1.07
	Modified CTAB 1	Amin	511.84	0.80	2.03	0.97
		Conservolia	254.56	0.43	2.06	0.74
		Megaron	261.4	0.33	2.04	1.18
		Grossane	287.28	0.17	2.06	1.38
	Modified CTAB 2	Meshkat	42	0.94	1.39	0.70
		Amin	19.81	0.57	1.08	0.41
		Arbequina	11.2	0	1.60	0.24
		Dire	18.7	0.16	1.20	0.51
Air-dried extracted RNA	TRIzol 1	Amin	741.933	0.65	2.05	1.21
		Tokhm-e-Kabki	599.28	0.14	1.99	1.37
		Conservolia	608.14	0.04	1.96	0.99
		X3	25.99	0	2.03	1.97
	TRIzol 2	Amin	210.73	0.97	1.91	0.64
		Tokhm-e-Kabki	36.91	0.85	1.89	0.57
		Cornicabra	216.98	0.88	1.68	0.59
		Dire	80.49	0.64	1.86	0.57
	Modified CTAB 1	Amin	377.56	0.89	2.10	1.11
		Grossane	8.87	0	2.17	0.93
		Meshkat	102.63	0.68	1.99	0.96
		Halkidikis	218.51	0.73	1.40	1.16
	Ribospin plant RNA extraction kit	Koroneiki	11.55	0.96	1.96	1.50
Complementary techniques	Lyophilized leaves	Dire	282.89	0.46	1.91	0.64
		Amin	20.61	0.56	1.88	0.95
		Meshkat	59.29	0.80	1.88	0.25
		Arbequina	137.70	0.64	2.06	0.76
	Storing in RNAlater	Amin	526.23	0.27	2.09	1.61
		Meshkat	681.46	0.32	2.09	1.30
		Tokhm-e-Kabki	494.26	0.16	2.03	1.61
		Dire	497.88	0.16	2.05	1.53

ISSN: 1746-4811