Fig. 1
From: Stability investigation of air-dried olive ribo nucleic acids for metavirome studies
Assessment of RNA quality by electrophoresis, the synthesized cDNAs quality for amplification of housekeeping genes (Rbc 1 and Nad 5), the prepared libraries, and partial amplification of viral genomes by RT-PCR. a Electrophoresis of RNAs on 1.5% agarose gel extracted from fresh tissues of olive cultivars ‘Dire’, ‘Meshkat’, ‘Conservolia’, ‘Arbequina’. b, c Partial amplification of 184 bp and 181 bp genomic fragments of Rbc 1 and Nad 5 genes, respectively using the cDNAs that synthesized from TRIzol extracted RNAs of cultivars ‘Dire’, ‘Meshkat’, ‘Conservolia’ and ‘Arbequina’. d Electrophoresis pattern of libraries prepared according to NEBNext® Ultra™ II multiplex small RNA Library Prep set (NEW ENGLAND BioLabs, USA) for cultivars ‘Dire’ and ‘Meshkat’ on 6% polyacrylamide mini-protecan procase gel (Bio-Rad, USA). e RT-PCR detection of Arabis mosaic virus by amplification of the viral 427 bp genome in cultivars ‘Arbequina’, ‘Amin’, ‘Meshkat’, ‘Conservolia’, ‘Tokhm-e-Kabki’, ‘Dire’, ‘KH15’, ‘Shenge’, negative (2) and positive (3) control; f Partial amplification of a 513 bp fragment belonging to Cucumber mosaic virus genome in cultivars ‘Mastoidis’, ‘Zard’, ‘Meshkat’, ‘Amygdalolia’, ‘KH15’, ‘Coratina’, ‘Dire’, negative (2) and positive (3) controls. Lanes (5) in figure a and 2 in b, c, e, f indicate negative controls, and lanes 3 in e and f show positive controls. DNA ladders are 1 Kb plus ladder (Fermentas, USA) for a, b and c; Invitrogen 10 bp DNA ladder for d and 100 bp ladder for e and f