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Fig. 2 | Plant Methods

Fig. 2

From: An efficient overexpression method for studying genes in Ricinus that transport vectorized agrochemicals

Fig. 2

Optimization of overexpression parameters. A1A4 Observation of fluorescence strength by laser confocal microscopy in merged field at 24 h, 48 h, 72 h, and 96 h. B Relative expression level of eGFP after 24 h, 48 h, 72 h, and 96 h, detected respectively. C Optimization of Agrobacterium concentration. Series Agrobacterium cell densities, including OD600 at 0.4, 0.8, 1.2, and 1.6, were used. D Optimization of infiltration time. The duration of vacuum infiltration was set at 10 min, 20 min, 20 plus 20 min and 40 min. All data were collected and handled from three biological replicates. Different samples were used during these biological replicates. Letters on the graphs denote statistically significant differences (ANOVA, P < 0.05)

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