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Fig. 6 | Plant Methods

Fig. 6

From: An advanced method for the release, enrichment and purification of high-quality Arabidopsis thaliana rosette leaf trichomes enables profound insights into the trichome proteome

Fig. 6

Comparison of A. thaliana leaf tissue and trichomes regarding AtEXO70H4 expression and proteome composition. A Transcript abundance of AtEXO70H4 in leaf and trichome samples. AtEXO70H4 transcript levels were determined by qRT-PCR using the 2−∆∆CT method. Dots represent the mean of three replicates in a single experiment relative to AtACT2 or AtTUB4 expression. Lines indicate the mean of AtEXO70H4 expression regarding the two reference genes. B Total protein extracts of leaf and trichome samples after SDS-PAGE and SYPRO Ruby gel staining. Red arrowheads mark more intense signals upon comparison of LEAF(−) and PURE samples. C Proteins identified in trichome PURE samples referred to LEAF(−) samples depending on the respective p-values (Student’s t-test). Top ten enriched/depleted proteins were tagged by name. Dots represent proteins identified in at least three of four measured samples. Vertical dashed lines indicate the borders for two-fold enrichment or depletion. Coloration of the dots highlights proteins significantly and at least two-fold enriched or depleted in PURE compared to LEAF(−) samples. "Others" refers to proteins that were not assigned to one of the two categories. LEAF(+): A. thaliana leaves harboring trichomes, LEAF(−): Leaves after trichome release, RAW: Trichomes before DGC, PURE: Trichomes after sucrose DGC

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