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Fig. 5 | Plant Methods

Fig. 5

From: An advanced method for the release, enrichment and purification of high-quality Arabidopsis thaliana rosette leaf trichomes enables profound insights into the trichome proteome

Fig. 5

Analysis of the cell wall composition of A. thaliana trichomes. The amount of cell wall matrix monosaccharides and crystalline cellulose in trichomes was determined. RAW samples and trichomes purified by a Nycodenz DGC were included, to assess the impact of residual glucose in trichome samples after sucrose DCG. Columns indicate the result of a single experiment. A Amount of cell wall monosaccharides in the alcohol insoluble residue (in mg) recovered by the indicated release and purification strategies. B Total amount of all matrix wall monosaccharides in the alcohol insoluble residue (in mg) of the various samples. C Intra-method variation of monosaccharide amounts among trichomes processed by the indicated methods. Dots represent the CoV of the four values presented for each method in panel A. D Amount of cellulosic glucose per trichome in the alcohol insoluble residue (in mg) recovered by the indicated release and purification strategies. Values in panels A, B and D are given per mg alcohol insoluble residue

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