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Fig. 3 | Plant Methods

Fig. 3

From: An advanced method for the release, enrichment and purification of high-quality Arabidopsis thaliana rosette leaf trichomes enables profound insights into the trichome proteome

Fig. 3

Isolation of trichomes from other plant species. Trichomes were released from S. lycopersicum, S. tuberosum, N. benthamiana and H. annuus by the STIRRER method and enriched as described for A. thaliana. Please note that due to the leaf size of these plants we reduced the amount of processed plant material to about 15 g per sample to ensure free floating of the leaves in the EDTA-PBS buffer. Micrographs show the adaxial side of leaves before (LEAF(+)) and after (LEAF(−)) trichome release as well as detached trichomes after enrichment (RAW). Arrowheads in the LEAF(−) micrographs mark trichomes that remained attached to the leaf surface. Reaction tubes show total trichome yield per plant species. Brightfield microscopy of enriched trichomes was performed with a 50 µL sample of the 1 mL trichome suspension. Scale bars represent 100 µm

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Plant Methods

ISSN: 1746-4811